This retrospective study included 299 patients with type 2 diabetes mellitus and diabetic nephropathy biopsy confirmed (DN) to investigate the prognostic value of alkaline phosphatase (ALP) for kidney outcomes. Cox proportional hazards models were used to estimate hazard ratios (HR) for serum levels of ALP on renal outcomes, defined as end-stage renal disease (ESRD) or a 50% reduction in estimated glomerular filtration rate (eGFR) from baseline. ALP baseline average was 80 IU / L with various interquartile range from 64-97 IU / L Serum ALP negatively related to eGFR but positively associated with proteinuria and renal interstitial fibrosis.

During a median follow-up period of 23 months, ESRD or 50% decrease in eGFR occurred in 156 (52.2%) patients. ALP highest quartile were significantly associated with poor renal outcomes, as defined above (HR 2.38, 95% confidence interval [CI] 1.09 to 5.17), after adjusting for sociodemographic factors, basic eGFR, proteinuria, parameters of liver function, parathyroid hormone levels, and renal pathological findings.

Each standard deviation higher in natural log-transformed ALP is associated with an increased risk of 25% for poor renal outcome. In addition, there is a graded increase in the risk for poor renal outcomes with ALP was higher in patients with nephrotic-range proteinuria. However, no significant correlation was observed between serum levels of ALP and renal outcomes in patients with non-nephrotic proteinuria-range. In conclusion, high ALP levels were independently associated with poor renal outcomes in patients with type 2 diabetes mellitus and nephrotic-range proteinuria after multivariate adjustment.

Alkaline phosphatase (ALP) is important in the diagnosis of various diseases. Because the ALP used to detect biomarkers for many diseases, many researchers conduct an investigation to develop detection strategies ALP. The use of fluorescent material has attracted attention because of the high sensitivity of the technique and low sample volume required.

Here, we review and discuss the mechanism of action and advantage of four main categories: DNA fluorescent probes, fluorescent molecular probes, probe-based chemistry of coordination, and probe nanoparticles. development prospects and trends are also discussed.

Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli

protein folding studies using the Force Profile Analysis Cotranslational, where SECM translation method used to detect the capture peptide folding due to the forces acting on the nascent polypeptide has so far been limited mainly to small domains cytosolic proteins that fold in close by translating ribosomes.

In this study, we investigated the cotranslational of periplasmic folding, disulfide bond containing proteins of E. coli alkaline phosphatase (Phoa) in a wild-type strain background and the background of the tension without periplasmic thiol: disulfide interchange protein DsbA.

p38
MBS8533658-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

p38
MBS8533658-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

p38
MBS8533658-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

P38 mAb
MBS435304-005mL	MyBiosource	0.05mL	EUR 415

P38 mAb
MBS435304-01mL	MyBiosource	0.1mL	EUR 660

P38 mAb
MBS435304-5x01mL	MyBiosource	5x0.1mL	EUR 2655

p38 MAPK
E8ET1702-65	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

Mxi2, p38
MBS603655-01mg	MyBiosource	0.1mg	EUR 820

Mxi2, p38
MBS603655-5x01mg	MyBiosource	5x0.1mg	EUR 3530

p38 MAPK
MBS8534372-01mL	MyBiosource	0.1mL	EUR 345

p38 MAPK
MBS8534372-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

p38 MAPK
MBS8534372-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

p38 MAPK
MBS8534372-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

p38 MAPK
MBS8534372-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

p38 protein
E20-80078	EnoGene	20ug	EUR 275
Description: N/A

p38 protein
E20-80079	EnoGene	20ug	EUR 275
Description: N/A

p38 protein
MBS8526800-002mg	MyBiosource	0.02mg	EUR 345

p38 protein
MBS8526800-5x002mg	MyBiosource	5x0.02mg	EUR 1410

p38 protein
MBS8526801-002mg	MyBiosource	0.02mg	EUR 345

p38 protein
MBS8526801-5x002mg	MyBiosource	5x0.02mg	EUR 1410

p38 Antibody
20-abx007541	Abbexa	Ask for price Ask for price Ask for price	30 ul 100 ul 200 ul

p38 antibody
70R-51505	Fitzgerald	100 ul	EUR 242
Description: Purified Polyclonal p38 antibody

p38 antibody
70R-51506	Fitzgerald	100 ul	EUR 286
Description: Purified Polyclonal p38 antibody

p38 Antibody
35478	SAB	100ul	EUR 479

p38 Antibody
35478-100ul	SAB	100ul	EUR 468

p38 Antibody
20-abx133223	Abbexa	Ask for price Ask for price Ask for price	30 ul 100 ul 200 ul

p38 Antibody
48644	SAB	100ul	EUR 499

p38 Antibody
48644-100ul	SAB	100ul	EUR 399.6

p38 Antibody
48644-50ul	SAB	50ul	EUR 286.8

p38 Antibody
20-abx141038	Abbexa	Ask for price Ask for price Ask for price	30 ul 100 ul 200 ul

p38 Antibody
B7178-100ul	Assay Biotech	100μl	EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B7178-50ul	Assay Biotech	50μl	EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-100ul	Assay Biotech	100μl	EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-50ul	Assay Biotech	50μl	EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-100ul	Assay Biotech	100μl	EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-50ul	Assay Biotech	50μl	EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
abx236076-100g	Abbexa	100 µg	EUR 350

p38 Antibody
abx236076-100ug	Abbexa	100 ug	EUR 577.2

p38 Antibody
E38PA4721	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5060	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5094	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA7126	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA3186	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Antibody
MBS5316121-01mL	MyBiosource	0.1mL	EUR 460

p38 Antibody
MBS5316121-5x01mL	MyBiosource	5x0.1mL	EUR 1920

p38 Antibody
MBS5316122-01mL	MyBiosource	0.1mL	EUR 535

p38 Antibody
MBS5316122-5x01mL	MyBiosource	5x0.1mL	EUR 2270

p38 Antibody
MBS8002013-0025mL	MyBiosource	0.025mL	EUR 315

p38 Antibody
MBS8002013-5x0025mL	MyBiosource	5x0.025mL	EUR 890

p38 Antibody
MBS805089-01mL	MyBiosource	0.1mL	EUR 425

p38 Antibody
MBS805089-5x01mL	MyBiosource	5x0.1mL	EUR 1350

p38 Antibody
MBS806203-0025mL	MyBiosource	0.025mL	EUR 320

p38 Antibody
MBS8581787-01mL	MyBiosource	0.1mL	EUR 305

p38 Antibody
MBS8581787-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

p38 Antibody
MBS8581787-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

p38 Antibody
MBS8581787-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

p38 Antibody
MBS8581787-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

p38 Antibody
MBS8584173-01mL	MyBiosource	0.1mL	EUR 305

p38 Antibody
MBS8584173-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

p38 Antibody
MBS8584173-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

p38 Antibody
MBS8584173-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

p38 Antibody
MBS8584173-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

p38 Antibody
MBS8582665-01mL	MyBiosource	0.1mL	EUR 305

p38 Antibody
MBS8582665-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

p38 Antibody
MBS8582665-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

p38 Antibody
MBS8582665-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

p38 Antibody
MBS8582665-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

p38 Antibody
MBS8582905-01mL	MyBiosource	0.1mL	EUR 305

p38 Antibody
MBS8582905-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

p38 Antibody
MBS8582905-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

p38 Antibody
MBS8582905-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

p38 Antibody
MBS8582905-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

p38 Antibody
MBS8582923-01mL	MyBiosource	0.1mL	EUR 305

p38 Antibody
MBS8582923-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

p38 Antibody
MBS8582923-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

p38 Antibody
MBS8582923-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

p38 Antibody
MBS8582923-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

p38 Antibody
MBS9508977-005mL	MyBiosource	0.05mL	EUR 285

p38 Antibody
MBS9508977-01mL	MyBiosource	0.1mL	EUR 385

p38 Antibody
MBS9508977-5x01mL	MyBiosource	5x0.1mL	EUR 1590

p38 Antibody
MBS9409774-01mL	MyBiosource	0.1mL	EUR 420

p38 Antibody
MBS9409774-5x01mL	MyBiosource	5x0.1mL	EUR 1740

p38 Antibody
MBS9435492-005mL	MyBiosource	0.05mL	EUR 325

p38 Antibody
MBS9435492-01mL	MyBiosource	0.1mL	EUR 435

p38 Antibody
MBS9435492-5x01mL	MyBiosource	5x0.1mL	EUR 1810

p38 Antibody
SPC-172D	Stressmarq	0.1ml	EUR 161
Description: Rabbit Anti-Human p38 Polyclonal

p38 Antibody
SPC-172S	Stressmarq	0.012ml	Ask for price
Description: Rabbit Anti-Human p38 Polyclonal

p38 Substrate
MBS517838-1mg	MyBiosource	1mg	EUR 205

p38 Substrate
MBS517838-5x1mg	MyBiosource	5x1mg	EUR 675

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-10Blots	MyBiosource	10Blots	EUR 430

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-5x10Blots	MyBiosource	5x10Blots	EUR 1785

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-01mL	MyBiosource	0.1mL	EUR 695

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-5x01mL	MyBiosource	5x0.1mL	EUR 2985

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-02mL	MyBiosource	0.2mL	EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-5x02mL	MyBiosource	5x0.2mL	EUR 2810

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-02mL	MyBiosource	0.2mL	EUR 650

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-06mL	MyBiosource	0.6mL	EUR 1175

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-5x06mL	MyBiosource	5x0.6mL	EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-10Blots	MyBiosource	10Blots	EUR 875

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-5x10Blots	MyBiosource	5x10Blots	EUR 3785

SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor
E1B1863	EnoGene	1mg	EUR 85

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-1mg	MyBiosource	1mg	EUR 180

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-5x1mg	MyBiosource	5x1mg	EUR 660

p38 (411-425) M.leprae
MBS8246858-10mg	MyBiosource	10mg	EUR 675

p38 (411-425) M.leprae
MBS8246858-1mg	MyBiosource	1mg	EUR 255

p38 (411-425) M.leprae
MBS8246858-5mg	MyBiosource	5mg	EUR 480

p38 (411-425) M.leprae
MBS8246858-5x10mg	MyBiosource	5x10mg	EUR 2955

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-04mL	MyBiosource	0.4mL	EUR 670

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-5x04mL	MyBiosource	5x0.4mL	EUR 2870

Anti - p38-a
MBS684252-002mL	MyBiosource	0.02mL	EUR 270

Anti - p38-a
MBS684252-005mL	MyBiosource	0.05mL	EUR 485

Anti - p38-a
MBS684252-01mL	MyBiosource	0.1mL	EUR 720

Anti - p38-a
MBS684252-5x01mL	MyBiosource	5x0.1mL	EUR 2925

Anti - p38-b
MBS684253-002mL	MyBiosource	0.02mL	EUR 270

Anti - p38-b
MBS684253-005mL	MyBiosource	0.05mL	EUR 485

Anti - p38-b
MBS684253-01mL	MyBiosource	0.1mL	EUR 720

Anti - p38-b
MBS684253-5x01mL	MyBiosource	5x0.1mL	EUR 2925

Anti - p38-g
MBS684254-002mL	MyBiosource	0.02mL	EUR 270

Anti - p38-g
MBS684254-005mL	MyBiosource	0.05mL	EUR 485

Anti - p38-g
MBS684254-01mL	MyBiosource	0.1mL	EUR 720

Anti - p38-g
MBS684254-5x01mL	MyBiosource	5x0.1mL	EUR 2925

Anti - p38-d
MBS684255-002mL	MyBiosource	0.02mL	EUR 270

Anti - p38-d
MBS684255-005mL	MyBiosource	0.05mL	EUR 485

Anti - p38-d
MBS684255-01mL	MyBiosource	0.1mL	EUR 720

Anti - p38-d
MBS684255-5x01mL	MyBiosource	5x0.1mL	EUR 2925

p38 Mouse mAb
E2220879	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb
E2222580	EnoGene	100ul	EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb
MBS8539086-01mL	MyBiosource	0.1mL	EUR 305

p38 Mouse mAb
MBS8539086-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

p38 Mouse mAb
MBS8539086-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

p38 Mouse mAb
MBS8539086-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

p38 Mouse mAb
MBS8539086-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

We found that the folding-induced forces can be transmitted through the nascent chain of periplasmic polypeptides to transferase center on the ribosome, a distance of ~ 160 å, and that appears to fold cotranslationally Phoa through at least two disulfide-stabilized folding intermediates. Thus, the Force Profile Analysis can be used to study protein folding cotranslational in extra-cytosolic compartment, such as periplasm. This article is protected by copyright. All rights reserved.