Association between serum alkaline phosphatase and renal outcome in patients with type 2 diabetes mellitus

This retrospective study included 299 patients with type 2 diabetes mellitus and diabetic nephropathy biopsy confirmed (DN) to investigate the prognostic value of alkaline phosphatase (ALP) for kidney outcomes. Cox proportional hazards models were used to estimate hazard ratios (HR) for serum levels of ALP on renal outcomes, defined as end-stage renal disease (ESRD) or a 50% reduction in estimated glomerular filtration rate (eGFR) from baseline. ALP baseline average was 80 IU / L with various interquartile range from 64-97 IU / L Serum ALP negatively related to eGFR but positively associated with proteinuria and renal interstitial fibrosis.

During a median follow-up period of 23 months, ESRD or 50% decrease in eGFR occurred in 156 (52.2%) patients. ALP highest quartile were significantly associated with poor renal outcomes, as defined above (HR 2.38, 95% confidence interval [CI] 1.09 to 5.17), after adjusting for sociodemographic factors, basic eGFR, proteinuria, parameters of liver function, parathyroid hormone levels, and renal pathological findings.

Each standard deviation higher in natural log-transformed ALP is associated with an increased risk of 25% for poor renal outcome. In addition, there is a graded increase in the risk for poor renal outcomes with ALP was higher in patients with nephrotic-range proteinuria. However, no significant correlation was observed between serum levels of ALP and renal outcomes in patients with non-nephrotic proteinuria-range. In conclusion, high ALP levels were independently associated with poor renal outcomes in patients with type 2 diabetes mellitus and nephrotic-range proteinuria after multivariate adjustment.

Alkaline phosphatase (ALP) is important in the diagnosis of various diseases. Because the ALP used to detect biomarkers for many diseases, many researchers conduct an investigation to develop detection strategies ALP. The use of fluorescent material has attracted attention because of the high sensitivity of the technique and low sample volume required.

Here, we review and discuss the mechanism of action and advantage of four main categories: DNA fluorescent probes, fluorescent molecular probes, probe-based chemistry of coordination, and probe nanoparticles. development prospects and trends are also discussed.

Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli

protein folding studies using the Force Profile Analysis Cotranslational, where SECM translation method used to detect the capture peptide folding due to the forces acting on the nascent polypeptide has so far been limited mainly to small domains cytosolic proteins that fold in close by translating ribosomes.

In this study, we investigated the cotranslational of periplasmic folding, disulfide bond containing proteins of E. coli alkaline phosphatase (Phoa) in a wild-type strain background and the background of the tension without periplasmic thiol: disulfide interchange protein DsbA.

p38

MBS8533658-01mLAF405S 0.1mL(AF405S)
EUR 565

p38

MBS8533658-01mLAF610 0.1mL(AF610)
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p38

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P38 mAb

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P38 mAb

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P38 mAb

MBS435304-5x01mL 5x0.1mL
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p38 MAPK

E8ET1702-65 100ul
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Description: Available in various conjugation types.

Mxi2, p38

MBS603655-01mg 0.1mg
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Mxi2, p38

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p38 MAPK

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p38 MAPK

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p38 MAPK

MBS8534372-01mLAF635 0.1mL(AF635)
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p38 protein

E20-80078 20ug
EUR 275
Description: N/A

p38 protein

E20-80079 20ug
EUR 275
Description: N/A

p38 protein

MBS8526800-002mg 0.02mg
EUR 345

p38 protein

MBS8526800-5x002mg 5x0.02mg
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p38 protein

MBS8526801-002mg 0.02mg
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p38 protein

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p38 Antibody

20-abx141038
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p38 Antibody

20-abx133223
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p38 Antibody

20-abx007541
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p38 Antibody

48644 100ul
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p38 Antibody

48644-100ul 100ul
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p38 Antibody

48644-50ul 50ul
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p38 Antibody

35478 100ul
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abx236076-100ug 100 ug
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p38 Antibody

E38PA4721 100ul
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p38 Antibody

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Description: Available in various conjugation types.

p38 Antibody

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p38 Antibody

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Description: Available in various conjugation types.

p38 Antibody

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p38 antibody

70R-51505 100 ul
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Description: Purified Polyclonal p38 antibody

p38 antibody

70R-51506 100 ul
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p38 Antibody

abx236076-100g 100 µg
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p38 Antibody

MBS9409774-01mL 0.1mL
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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

MBS8584173-01mLAF405L 0.1mL(AF405L)
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p38 Antibody

MBS8584173-01mLAF405S 0.1mL(AF405S)
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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

MBS8582905-01mLAF635 0.1mL(AF635)
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p38 Antibody

MBS8582923-01mL 0.1mL
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p38 Antibody

MBS8582923-01mLAF405L 0.1mL(AF405L)
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p38 Antibody

MBS8582923-01mLAF405S 0.1mL(AF405S)
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p38 Antibody

MBS8582923-01mLAF610 0.1mL(AF610)
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p38 Antibody

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p38 Antibody

MBS8581787-01mL 0.1mL
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p38 Antibody

MBS8581787-01mLAF405L 0.1mL(AF405L)
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p38 Antibody

MBS8581787-01mLAF405S 0.1mL(AF405S)
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p38 Antibody

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p38 Antibody

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p38 Antibody

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p38 Antibody

MBS5316121-5x01mL 5x0.1mL
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p38 Antibody

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p38 Antibody

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p38 Antibody

MBS9435492-005mL 0.05mL
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p38 Antibody

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p38 Antibody

MBS9435492-5x01mL 5x0.1mL
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p38 Antibody

MBS9508977-005mL 0.05mL
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p38 Antibody

MBS9508977-01mL 0.1mL
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p38 Antibody

MBS9508977-5x01mL 5x0.1mL
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p38 Antibody

B0798-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0798-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-100ul 100μl
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Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B7178-100ul 100μl
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Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B7178-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Substrate

MBS517838-1mg 1mg
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p38 Substrate

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MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS657552-10Blots 10Blots
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MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

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MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-02mL 0.2mL
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MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-06mL 0.6mL
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MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-5x06mL 5x0.6mL
EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-02mL 0.2mL
EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-5x02mL 5x0.2mL
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MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-01mL 0.1mL
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MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-5x01mL 5x0.1mL
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MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS627818-10Blots 10Blots
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MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

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SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor

E1B1863 1mg
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SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

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SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

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p38 (411-425) M.leprae

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p38 (411-425) M.leprae

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p38 (411-425) M.leprae

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MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)

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Anti - p38-a

MBS684252-002mL 0.02mL
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Anti - p38-a

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Anti - p38-a

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Anti - p38-d

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Anti - p38-d

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Anti - p38-d

MBS684255-01mL 0.1mL
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Anti - p38-d

MBS684255-5x01mL 5x0.1mL
EUR 2925

p38 Mouse mAb

E2220879 100ul
EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb

E2222580 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb

MBS8539086-01mL 0.1mL
EUR 305

p38 Mouse mAb

MBS8539086-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF610 0.1mL(AF610)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

E2340697 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2380881 100ul
EUR 225
Description: Available in various conjugation types.

We found that the folding-induced forces can be transmitted through the nascent chain of periplasmic polypeptides to transferase center on the ribosome, a distance of ~ 160 å, and that appears to fold cotranslationally Phoa through at least two disulfide-stabilized folding intermediates. Thus, the Force Profile Analysis can be used to study protein folding cotranslational in extra-cytosolic compartment, such as periplasm. This article is protected by copyright. All rights reserved.