Synthesis, characterization, alkaline phosphatase inhibition assay and molecular modeling studies of 1-benzylidene-2-(4-tert- butylthiazol-2-yl) hydrazines

Synthesis, characterization, alkaline phosphatase inhibition assay and molecular modeling studies of 1-benzylidene-2-(4-tert- butylthiazol-2-yl) hydrazines

alkaline phosphatase is an enzyme that removes phosphate homodimeric proteins of several types of molecules. This catalyzes the hydrolysis of monoesters in phosphoric acid, which in turn catalyzes transfosforilasi. Thiazoles is a special class of heterocyclic compounds that could potentially be an effective phosphatase inhibitors. In this case, this research paper reported the synthesis and characterization smoothly substituted 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine with excellent results.

Synthesized compounds tested for potency inhibition of alkaline phosphatase. The compound 1- (4-hydroxy, 3-methoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine (<b> 5e) </ b> was found to be the most potent inhibitor of human tissue non phosphatase -alkaline in this group of molecules with IC <sub> 50 </ sub> value of 1.09 ± 0.18 pM. The compound 1- (3,4-dimethoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine <b> (5d) </ b> exhibited selectivity and the potential for human intestinal alkaline phosphatase with IC <sub> 50 </ sub> value of 0.71 ± 0.02 pM.

In addition, the structure activity relationship and molecular docking studies were performed to evaluate their mode of binding to the target site alkaline phosphatase. Docking analysis revealed that the most active inhibitor showed significant interaction in the binding pocket of human intestinal alkaline phosphatase and alkaline phosphatase non-human tissues and may be responsible for the inhibitory activity of compounds against the enzyme. Therefore, the filtered thiazole derivatives provided a superb platform for the further development of alkaline phosphatase inhibitor.

Alkaline phosphatase assay revealed the compound <b> 5e </ b> (1- (4-hydroxy, 3-methoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine) as the most active inhibitor <b> < i> h </ i> -TNAP </ b> IC <sub> 50 </ sub> value of 1.09 ± 0.18 pM. Computing evaluation clearly illustrates the multiple interactions in the binding pocket <b> <i> h </ i> -IAP </ b> and <b> <i> h </ i> -TNAP </ b> and may be responsible for potential inhibitory compounds against the enzyme. Highlights Synthesis of 1- (benzylidene) thiosemicarbazides <b> 3 (a-i) </ b> reacting it with substituted aromatic aldehydes

Thiosemicarbazide <b> 1 (a-i) </ b>. synthesized 1- (benzylidene) thiosemicarbazides was cyclized with 1-chloropinacolone to get each 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine <b> 5 (a-i) </ b>. synthesized 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine <b> 5 (a-i) </ b> successfully characterized using elemental analysis, FT-IR and multi nuclear NMR. Alkaline phosphatase assay and computational studies conducted in support of 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine synthesized <b> 5 (a-i) </ b>.

Reduce background absorption through the double-key strategy for the detection of alkaline phosphatase and α-fetoprotein

Lowering the background signal for analysis is more sensitive than determinands equally important to strengthen the target signal. Oxidase photoinduced of fluorescein have been reported, which can catalyze the oxidation of a common substrate in a few minutes. As a metaphor for the lock and key, we designed a double lock limiting the activity of fluorescein to reduce background uptake during colorimetric detection.

StemTAG Alkaline Phosphatase Activity Assay Kit, Fluorometric

CBA-307 100 assays
EUR 540
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Acid Phosphatase Activity Fluorometric Assay Kit

K421-500 each
EUR 418.8

Alkaline Phosphatase Activity Colorimetric Assay Kit

K2075-500 500 assays
EUR 652.8

Alkaline Phosphatase Activity Colorimetric Assay Kit

K412-500 each
EUR 646.8

Genorise® Alkaline Phosphatase Activity Fluorometric Assay Kit

GR107016 100 tests
EUR 346.8

mPEG-SH,500

33-MF001003-500
  • EUR 310.80
  • EUR 812.40
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-COOH?500

33-MF001017-500
  • EUR 237.60
  • EUR 592.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-MAL,500

33-MF001022-500
  • EUR 282.00
  • EUR 726.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Alkaline Phosphatase Activity Assay Kit

55R-1402 500 assays
EUR 888
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit

55R-1406 500 assays
EUR 525.6
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Fluorometric

CBA-308 2 x 100 assays
EUR 846
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

HO-PEG-COOH,500

33-HE002017-500
  • EUR 237.60
  • EUR 592.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-COOH,500

33-HE003017-500
  • EUR 622.80
  • EUR 1747.20
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

HS-PEG-NHS,500

33-HE003023-500
  • EUR 534.00
  • EUR 1480.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-COOH,500

33-HE005017-500
  • EUR 430.80
  • EUR 1170.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-OH,500

33-HE006002-500
  • EUR 681.60
  • EUR 1923.60
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-BIOTIN,500

33-HE006041-500
  • EUR 992.40
  • EUR 2856.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Biotin-PEG-COOH,500

33-HE041017-500
  • EUR 726.00
  • EUR 2056.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-SH,500

33-HO003003-500
  • EUR 310.80
  • EUR 812.40
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

AlKyne-PEG-AlKyne,500

33-HO007007-500
  • EUR 339.60
  • EUR 896.40
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

COOH-PEG-COOH,500

33-HO017017-500
  • EUR 200.40
  • EUR 478.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SC-PEG-SC,500

33-HO023023-500
  • EUR 200.40
  • EUR 478.80
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)

K829-100 each
EUR 639.6

Alkaline Phosphatase Colorimetric Activity Kit (2 Plate)

K082-H1 2x96 well plate
EUR 289

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric

CBA-301 100 assays
EUR 483.6
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Acid Phosphatase Activity Colorimetric Assay Kit

K411-500 each
EUR 430.8

Genorise® Alkaline Phosphatase Activity Colorimetric Assay Kit

GR107015 100 tests
EUR 346.8

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 380.4

Neuraminidase Activity Fluorometric Assay Kit

K2230-100 100 assays
EUR 718.8

HDAC Activity Fluorometric Assay Kit

K2031-100 100 assays
EUR 602.4

HAT Activity Fluorometric Assay Kit

K2034-100 100 assays
EUR 668.4

Calpain Activity Fluorometric Assay Kit

K2062-100 100 assays
EUR 752.4

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 668.4

Proteasome Activity Fluorometric Assay Kit

K2096-100 100 assays
EUR 602.4

GST Fluorometric Activity Assay Kit

K2105-100 100 assays
EUR 602.4

PLTP Activity Fluorometric Assay Kit

K2087-100 100 assays
EUR 802.8

CETP Activity Fluorometric Assay Kit

K2089-100 100 assays
EUR 870

Glucosylceramidase Activity Assay Kit (Fluorometric)

K2003-100 each
EUR 705.6

Transketolase Activity Assay Kit (Fluorometric)

K2004-100 each
EUR 744

Carboxylesterase Activity Assay Kit (Fluorometric)

K2014-100 each
EUR 744

Lysozyme Activity Assay Kit (Fluorometric)

K236-100 each
EUR 588

Calpain Activity Fluorometric Assay Kit

K240-100 each
EUR 705.6

Proteasome Activity Fluorometric Assay Kit

K245-100 each
EUR 574.8

GST Fluorometric Activity Assay Kit

K260-100 each
EUR 561.6

Sirtuin Activity Assay Kit (Fluorometric)

K324-100 each
EUR 686.4

HDAC Activity Fluorometric Assay Kit

K330-100 each
EUR 588

HAT Activity Fluorometric Assay Kit

K334-100 each
EUR 646.8

HDAC3 Activity Fluorometric Assay Kit

K343-100 each
EUR 574.8

HDAC8 Activity Fluorometric Assay Kit

K348-100 each
EUR 588

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100 each
EUR 588

Thrombin Activity Fluorometric Assay Kit

K373-100 each
EUR 836.4

Plasmin Activity Assay Kit (Fluorometric)

K381-100 each
EUR 548.4

HDAC6 Activity Assay Kit (Fluorometric)

K466-100 each
EUR 757.2

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100 each
EUR 639.6

Neprilysin Activity Assay Kit (Fluorometric)

K487-100 each
EUR 732

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100 each
EUR 744

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100 each
EUR 744

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100 each
EUR 679.2

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100 each
EUR 548.4

Urokinase Activity Fluorometric Assay Kit

K728-100 each
EUR 652.8

Neuraminidase Activity Fluorometric Assay Kit

K732-100 each
EUR 627.6

Uricase Activity Assay Kit (Fluorometric)

K734-100 each
EUR 614.4

DPP4 Activity Fluorometric Assay Kit

K779-100 each
EUR 652.8

Protease Activity Fluorometric Assay Kit

K781-100 each
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Renin Activity Fluorometric Assay Kit

K800-100 each
EUR 627.6

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100 each
EUR 783.6

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100 each
EUR 705.6

MicroMolar ADP assay kit - 500 assays

MAD500K 500 assays
EUR 1060.58
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MAD reagent 1, 150 ul of 100 x MAD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of ADP using a standard black 384-well plate.

MicroMolar GDP assay kit - 500 assays

MGD500K 500 assays
EUR 1060.58
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MGD reagent 1, 150 ul of 100 x MGD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of GDP using a standard black 384-well plate.

MicroMolar UDP assay kit - 500 assays

MUD500K 500 assays
EUR 1060.58
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MUD reagent 1, 150 ul of 100 x MUD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of UDP using a standard black 384-well plate.

Alkaline Phosphatase Assay Kit

abx096002-100Assays 100 Assays
EUR 379.2

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 266.4

Alkaline Phosphatase Assay Kit

abx098406-Toshiba120R150ml4R250ml1 Toshiba 120; R1: 50ml×4 R2: 50ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit

abx098406-Toshiba40R150ml4R250ml1 Toshiba 40; R1: 50ml×4 R2: 50ml×1
EUR 266.4

Alkaline Phosphatase Assay Kit

Z5030033 250 assays
EUR 792
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Colorimetric

CBA-302 2 x 100 assays
EUR 769.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Beta-Secretase Activity Fluorometric Assay Kit

K2043-100 100 assays
EUR 769.2

Beta-Mannosidase Activity Assay Kit (Fluorometric)

K2045-100 100 assays
EUR 661.2

Cathepsin B Activity Fluorometric Assay Kit

K2151-100 100 assays
EUR 619.2

Cathepsin K Activity Fluorometric Assay Kit

K2152-100 100 assays
EUR 619.2

Cathepsin L Activity Fluorometric Assay Kit

K2153-100 100 assays
EUR 619.2

Cathepsin D Activity Fluorometric Assay Kit

K2154-100 100 assays
EUR 619.2

Cathepsin S Activity Fluorometric Assay Kit

K2155-100 100 assays
EUR 619.2

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K2169-100 100 assays
EUR 686.4

Catalase Activity Colorimetric/Fluorometric Assay Kit

K2177-100 100 assays
EUR 501.6

Lipase Activity Fluorometric Assay Kit III

K2095-100 100 assays
EUR 703.2

OxiSelect Catalase Activity Assay Kit, Fluorometric

STA-339 500 assays
EUR 908.4
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

Cathepsin B Activity Fluorometric Assay Kit

K140-100 each
EUR 614.4

Cathepsin K Activity Fluorometric Assay Kit

K141-100 each
EUR 614.4

Cathepsin L Activity Fluorometric Assay Kit

K142-100 each
EUR 614.4

Cathepsin D Activity Fluorometric Assay Kit

K143-100 each
EUR 614.4

Cathepsin S Activity Fluorometric Assay Kit

K144-100 each
EUR 614.4

Cathepsin H Activity Fluorometric Assay Kit

K145-100 each
EUR 574.8

Cathepsin E Activity Assay Kit (Fluorometric)

K165-100 each
EUR 764.4

Granzyme B Activity Fluorometric Assay Kit

K168-100 each
EUR 639.6

cAMP Phosphodiesterase Activity Assay Kit (Fluorometric)

K2013-100 each
EUR 744

NADH Oxidase Activity Assay Kit (Fluorometric)

K2019-100 each
EUR 705.6

Adenosine Deaminase Activity Assay Kit (Fluorometric)

K328-100 each
EUR 548.4

HAT (H4) Activity Fluorometric Assay Kit

K336-100 each
EUR 601.2

InSitu HDAC Activity Fluorometric Assay Kit

K339-100 each
EUR 574.8

Factor VIIIa Activity Assay Kit (Fluorometric)

K358-100 each
EUR 646.8

Beta-Secretase Activity Fluorometric Assay Kit

K360-100 each
EUR 744

Factor Xa Activity Fluorometric Assay Kit

K361-100 each
EUR 744

Factor IXa Activity Assay Kit (Fluorometric)

K364-100 each
EUR 588

Neutrophil Elastase Activity Assay kit (Fluorometric)

K383-100 each
EUR 502.8

?-Secretase (BACE1) Activity Assay Kit (Fluorometric)

K388-100 each
EUR 724.8

Phospholipase A2 Activity Assay Kit (Fluorometric)

K400-100 each
EUR 679.2

DNAse I Activity Assay Kit (Fluorometric)

K429-100 each
EUR 836.4

Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)

K446-100 each
EUR 561.6

Cytidine Deaminase Activity Assay Kit (Fluorometric)

K451-100 each
EUR 561.6

Diamine Oxidase Activity Assay Kit (Fluorometric)

K496-100 each
EUR 679.2

Oxalate Oxidase Activity Assay Kit (Fluorometric)

K509-100 each
EUR 574.8

?-L-Fucosidase Activity Assay Kit (Fluorometric)

K542-100 each
EUR 627.6

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

K549-100 each
EUR 502.8

PLTP Activity Fluorometric Assay Kit II

K593-100 each
EUR 705.6

CETP Activity Fluorometric Assay Kit II

K595-100 each
EUR 770.4

Enolase Activity Colorimetric/Fluorometric Assay Kit

K691-100 each
EUR 952.8

Lipoprotein Lipase Activity Fluorometric Assay Kit

K721-100 each
EUR 646.8

Lipase Activity Fluorometric Assay Kit III

K724-100 each
EUR 639.6

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K745-100 each
EUR 660

Asparaginase Activity Colorimetric/Fluorometric Assay Kit

K754-100 each
EUR 660

Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

K758-100 each
EUR 732

PicoProbe? Hexokinase Activity Assay Kit (Fluorometric)

K769-100 each
EUR 764.4

Peroxidase Activity Colorimetric/Fluorometric Assay Kit

K772-100 each
EUR 464.4

Catalase Activity Colorimetric/Fluorometric Assay Kit

K773-100 each
EUR 502.8

MMP-3 Activity Fluorometric Assay Kit

K783-100 each
EUR 614.4

Rat Renin Activity Fluorometric Assay Kit

K806-100 each
EUR 777.6

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

K807-100 each
EUR 1038

TEV Protease Activity Assay Kit (Fluorometric)

K842-100 each
EUR 601.2

Total Phosphodiesterase Activity Assay Kit (Fluorometric)

K927-100 each
EUR 738

Lysyl Oxidase Activity Assay Kit (Fluorometric)

K928-100 each
EUR 639.6

PicoProbeTM Glucokinase Activity Assay Kit (Fluorometric)

K969-100 each
EUR 711.6

Aromatase (CYP19A) Activity Assay Kit (Fluorometric)

K983-100 each
EUR 744

The first key inhibit the main activity of fluorescein by phosphate. The second key is almost completely disable fluorescein by forming nanoparticles coordination (CNPS) through self-assembly of cerium chloride and fluorescein diphosphate (FDP).