alkaline phosphatase is an enzyme that removes phosphate homodimeric proteins of several types of molecules. This catalyzes the hydrolysis of monoesters in phosphoric acid, which in turn catalyzes transfosforilasi. Thiazoles is a special class of heterocyclic compounds that could potentially be an effective phosphatase inhibitors. In this case, this research paper reported the synthesis and characterization smoothly substituted 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine with excellent results.

Synthesized compounds tested for potency inhibition of alkaline phosphatase. The compound 1- (4-hydroxy, 3-methoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine (<b> 5e) </ b> was found to be the most potent inhibitor of human tissue non phosphatase -alkaline in this group of molecules with IC <sub> 50 </ sub> value of 1.09 ± 0.18 pM. The compound 1- (3,4-dimethoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine <b> (5d) </ b> exhibited selectivity and the potential for human intestinal alkaline phosphatase with IC <sub> 50 </ sub> value of 0.71 ± 0.02 pM.

In addition, the structure activity relationship and molecular docking studies were performed to evaluate their mode of binding to the target site alkaline phosphatase. Docking analysis revealed that the most active inhibitor showed significant interaction in the binding pocket of human intestinal alkaline phosphatase and alkaline phosphatase non-human tissues and may be responsible for the inhibitory activity of compounds against the enzyme. Therefore, the filtered thiazole derivatives provided a superb platform for the further development of alkaline phosphatase inhibitor.

Alkaline phosphatase assay revealed the compound <b> 5e </ b> (1- (4-hydroxy, 3-methoxybenzylidene) -2- (4-tert-butylthiazol-2-yl) hydrazine) as the most active inhibitor <b> < i> h </ i> -TNAP </ b> IC <sub> 50 </ sub> value of 1.09 ± 0.18 pM. Computing evaluation clearly illustrates the multiple interactions in the binding pocket <b> <i> h </ i> -IAP </ b> and <b> <i> h </ i> -TNAP </ b> and may be responsible for potential inhibitory compounds against the enzyme. Highlights Synthesis of 1- (benzylidene) thiosemicarbazides <b> 3 (a-i) </ b> reacting it with substituted aromatic aldehydes

Thiosemicarbazide <b> 1 (a-i) </ b>. synthesized 1- (benzylidene) thiosemicarbazides was cyclized with 1-chloropinacolone to get each 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine <b> 5 (a-i) </ b>. synthesized 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine <b> 5 (a-i) </ b> successfully characterized using elemental analysis, FT-IR and multi nuclear NMR. Alkaline phosphatase assay and computational studies conducted in support of 1-benzylidene-2- (4-tert-butylthiazol-2-yl) hydrazine synthesized <b> 5 (a-i) </ b>.

Reduce background absorption through the double-key strategy for the detection of alkaline phosphatase and α-fetoprotein

Lowering the background signal for analysis is more sensitive than determinands equally important to strengthen the target signal. Oxidase photoinduced of fluorescein have been reported, which can catalyze the oxidation of a common substrate in a few minutes. As a metaphor for the lock and key, we designed a double lock limiting the activity of fluorescein to reduce background uptake during colorimetric detection.

Alkaline Phosphatase Activity Fluorometric Assay Kit
K422-500	Biovision	each	EUR 418.8

Genorise® Alkaline Phosphatase Activity Fluorometric Assay Kit
GR107016	Genorise Scientific	100 tests	EUR 289

StemTAG Alkaline Phosphatase Activity Assay Kit, Fluorometric
CBA-307	Cell Biolabs	100 assays	EUR 540
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Fluorometric
CBA-308	Cell Biolabs	2 x 100 assays	EUR 846
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Acid Phosphatase Activity Fluorometric Assay Kit
GWB-AXR233	GenWay Biotech	500 assays	Ask for price

Acid Phosphatase Activity Fluorometric Assay Kit
K421-500	Biovision	each	EUR 418.8

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)
K829-100	Biovision	each	EUR 639.6

Alkaline Phosphatase Activity Assay Kit
55R-1402	Fitzgerald	500 assays	EUR 600
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit
55R-1406	Fitzgerald	500 assays	EUR 367
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

HAT Activity Fluorometric Assay Kit
K2034-100	ApexBio	100 assays	EUR 739.2
Description: Kits|Chromatin/Epigenetics Kit#Chromatin/Epigenetics Kit|Histone Acetyltransferases Kit#Kits|Neurodegenerative disease Kit#Kits

HAT Activity Fluorometric Assay Kit
K334-100	Biovision	each	EUR 646.8

DPP4 Activity Fluorometric Assay Kit
K2178-100	ApexBio	100 assays	EUR 691.2
Description: Kits|Apoptosis Kit#Apoptosis Kit|DPP-4 Kit#Kits

HDAC Activity Fluorometric Assay Kit
K2031-100	ApexBio	100 assays	EUR 602.4

HDAC Activity Fluorometric Assay Kit
K2031-96	ApexBio	96 tests	EUR 617.6
Description: Kits|Chromatin/Epigenetics Kit#Chromatin/Epigenetics Kit|HDAC Kit#Kits

PLTP Activity Fluorometric Assay Kit
K2087-100	ApexBio	100 assays	EUR 1043.2
Description: Metabolism Kit|Lipids Metabolism Kit#Kits|Metabolism Kit#Kits

CETP Activity Fluorometric Assay Kit
K2089-100	ApexBio	100 assays	EUR 1056
Description: Metabolism Kit|Lipids Metabolism Kit#Kits|Metabolism Kit#Kits

DPP4 Activity Fluorometric Assay Kit
K779-100	Biovision	each	EUR 652.8

HDAC Activity Fluorometric Assay Kit
K330-100	Biovision	each	EUR 588

Phospholipase A2 Activity Assay Kit (Fluorometric)
K400-100	Biovision	each	EUR 679.2

Renin Activity Fluorometric Assay Kit
K800-100	Biovision	each	EUR 627.6

HDAC8 Activity Fluorometric Assay Kit
K348-100	Biovision	each	EUR 588

HDAC3 Activity Fluorometric Assay Kit
K343-100	Biovision	each	EUR 574.8

Calpain Activity Fluorometric Assay Kit
K2062-100	ApexBio	100 assays	EUR 995.2
Description: Proteases/Proteasome Kit|Calpains Kit#Kits|Proteases/Proteasome Kit#Kits

Calpain Activity Fluorometric Assay Kit
K240-100	Biovision	each	EUR 705.6

Thrombin Activity Fluorometric Assay Kit
K373-100	Biovision	each	EUR 836.4

Protease Activity Fluorometric Assay Kit
K781-100	Biovision	each	EUR 464.4

HAT (H4) Activity Fluorometric Assay Kit
K336-100	Biovision	each	EUR 601.2

Urokinase Activity Fluorometric Assay Kit
K728-100	Biovision	each	EUR 652.8

Proteasome Activity Fluorometric Assay Kit
K2096-100	ApexBio	100 assays	EUR 870.4
Description: Kits|Proteases/Proteasome Kit#Proteases/Proteasome Kit|Proteasome Kit#Kits

Proteasome Activity Fluorometric Assay Kit
K245-100	Biovision	each	EUR 574.8

MMP-3 Activity Fluorometric Assay Kit
K783-100	Biovision	each	EUR 614.4

PLTP Activity Fluorometric Assay Kit II
K593-100	Biovision	each	EUR 705.6

CETP Activity Fluorometric Assay Kit II
K595-100	Biovision	each	EUR 770.4

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-M-500Assays	Elabscience Biotech	500 Assays	EUR 380
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-M-96T	Elabscience Biotech	96T	EUR 15
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-M-each	Elabscience Biotech	each	Ask for price
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-S-100Assays	Elabscience Biotech	100Assays	EUR 15
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-S-500Assays	Elabscience Biotech	500 Assays	EUR 380
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit
E-BC-K091-S-each	Elabscience Biotech	each	Ask for price
Description: Enzyme Activity

Factor Xa Activity Fluorometric Assay Kit
K361-100	Biovision	each	EUR 744

Rat Renin Activity Fluorometric Assay Kit
K806-100	Biovision	each	EUR 777.6

Factor Xa Activity Fluorometric Assay Kit
Kit-0337	Creative BioMart	100 tests	EUR 1198.4
Description: -20°C

Total Phosphodiesterase Activity Assay Kit (Fluorometric)
K927-100	Biovision	each	EUR 738

Neuraminidase Activity Fluorometric Assay Kit
K2230-100	ApexBio	100 assays	EUR 876.8
Description: Kits|Microbiology & Virology Kit#Microbiology & Virology Kit|NA Kit#Kits

Neuraminidase Activity Fluorometric Assay Kit
K732-100	Biovision	each	EUR 627.6

Lipase Activity Fluorometric Assay Kit III
K724-100	Biovision	each	EUR 639.6

Lipase Activity Fluorometric Assay Kit III
K2095-100	ApexBio	100 assays	EUR 736
Description: Metabolism Kit|Lipids Metabolism Kit#Kits|Metabolism Kit#Kits

Caspae-9 Activity Fluorometric Assay Kit
RP10239K	Abclonal	1kit	EUR 556.17

GST Fluorometric Activity Assay Kit
GWB-AXR173	GenWay Biotech	100 assays	Ask for price

GST Fluorometric Activity Assay Kit
K2105-100	ApexBio	100 assays	EUR 915.2
Description: Kits|Metabolism Kit#Metabolism Kit|Oxidative Stress Kit#Kits

GST Fluorometric Activity Assay Kit
K260-100	Biovision	each	EUR 561.6

Granzyme B Activity Fluorometric Assay Kit
K168-100	Biovision	each	EUR 639.6

Granzyme B Activity Fluorometric Assay Kit
K2001-100	ApexBio	100 assays	EUR 571
Description: Detects Apoptosis & Necrosis within 10 min

InSitu HDAC Activity Fluorometric Assay Kit
K339-100	Biovision	each	EUR 574.8

HDAC Fluorometric Activity Assay Kit
GWB-AXR199	GenWay Biotech	100 assays	Ask for price

Catalase -CAT- Activity Fluorometric Assay Kit
E-BC-F006-500Assays	Elabscience Biotech	500 Assays	EUR 960
Description: Enzyme Activity

Catalase -CAT- Activity Fluorometric Assay Kit
E-BC-F006-96T	Elabscience Biotech	96T	EUR 3
Description: Enzyme Activity

Catalase -CAT- Activity Fluorometric Assay Kit
E-BC-F006-each	Elabscience Biotech	each	Ask for price
Description: Enzyme Activity

Cathepsin B Activity Fluorometric Assay Kit
K140-100	Biovision	each	EUR 614.4

Cathepsin K Activity Fluorometric Assay Kit
K141-100	Biovision	each	EUR 614.4

Cathepsin L Activity Fluorometric Assay Kit
K142-100	Biovision	each	EUR 614.4

Cathepsin D Activity Fluorometric Assay Kit
K143-100	Biovision	each	EUR 614.4

Cathepsin S Activity Fluorometric Assay Kit
K144-100	Biovision	each	EUR 614.4

Cathepsin H Activity Fluorometric Assay Kit
K145-100	Biovision	each	EUR 574.8

Cathepsin B Activity Fluorometric Assay Kit
K2151-100	ApexBio	100 assays	EUR 723.2
Description: Proteases/Proteasome Kit|Cathepsin Kit#Kits|Proteases/Proteasome Kit#Kits

Cathepsin K Activity Fluorometric Assay Kit
K2152-100	ApexBio	100 assays	EUR 940.8
Description: Proteases/Proteasome Kit|Cathepsin Kit#Kits|Proteases/Proteasome Kit#Kits

Cathepsin L Activity Fluorometric Assay Kit
K2153-100	ApexBio	100 assays	EUR 896
Description: Proteases/Proteasome Kit|Cathepsin Kit#Kits|Proteases/Proteasome Kit#Kits

Cathepsin D Activity Fluorometric Assay Kit
K2154-100	ApexBio	100 assays	EUR 723.2
Description: Proteases/Proteasome Kit|Cathepsin Kit#Kits|Proteases/Proteasome Kit#Kits

Cathepsin S Activity Fluorometric Assay Kit
K2155-100	ApexBio	100 assays	EUR 723.2
Description: Proteases/Proteasome Kit|Cathepsin Kit#Kits|Proteases/Proteasome Kit#Kits

Catalase (CAT) Activity Fluorometric Assay Kit
MBS2567662-500Tests	MyBiosource	500Tests	EUR 830

Catalase (CAT) Activity Fluorometric Assay Kit
MBS2567662-5x100Tests	MyBiosource	5x100Tests	EUR 3830

Catalase (CAT) Activity Fluorometric Assay Kit
MBS2567662-5x96Test	MyBiosource	5x96Test	EUR 1615

Catalase (CAT) Activity Fluorometric Assay Kit
MBS2567662-96Test	MyBiosource	96Test	EUR 355

Catalase (CAT) Activity Fluorometric Assay Kit
MBS2567662-96Tests	MyBiosource	96Tests	EUR 355

Sphingomyelinase Activity Fluorometric Assay Kit
K574-100	Biovision	each	EUR 548.4

Proteasome Activity Fluorometric Assay Kit I
RP10220K	Abclonal	1Kit	EUR 456.98

Proteasome Activity Fluorometric Assay Kit II
RP10221K	Abclonal	1Kit	EUR 651.82

HDAC6 Activity Assay Kit (Fluorometric)
K466-100	Biovision	each	EUR 757.2

Alkaline Phosphatase Activity Colorimetric Assay Kit
GWB-AXR230	GenWay Biotech	500 assays	Ask for price

Alkaline Phosphatase Activity Colorimetric Assay Kit
K2075-500	ApexBio	500 assays	EUR 857.6
Description: Metabolism Kit|ALP Kit#Kits|Metabolism Kit#Kits

Alkaline Phosphatase Activity Colorimetric Assay Kit
K412-500	Biovision	each	EUR 646.8

GST Fluorometric Activity Assay
KT-205	Kamiya Biomedical Company	100 tests	EUR 510

Sirtuin Activity Assay Kit (Fluorometric)
K324-100	Biovision	each	EUR 686.4

Plasmin Activity Assay Kit (Fluorometric)
K381-100	Biovision	each	EUR 548.4

Uricase Activity Assay Kit (Fluorometric)
K734-100	Biovision	each	EUR 614.4

Beta-Secretase Activity Fluorometric Assay Kit
K360-100	Biovision	each	EUR 744

Beta-Secretase Activity Fluorometric Assay Kit
K2043-100	ApexBio	100 assays	EUR 1152
Description: Neurodegenerative disease Kit|Alzheimer disease Kit#Kits

Caspase-8, 10 Activity Fluorometric Assay Kit
RP10238K	Abclonal	1kit	EUR 556.17

Caspase-3, 7 Activity Fluorometric Assay Kit
RP10240K	Abclonal	1kit	EUR 556.17

Lysozyme Activity Assay Kit (Fluorometric)
K236-100	Biovision	each	EUR 588

Lipoprotein Lipase Activity Fluorometric Assay Kit
K721-100	Biovision	each	EUR 646.8

Neprilysin Activity Assay Kit (Fluorometric)
K487-100	Biovision	each	EUR 732

?-Xylosidase Activity Assay Kit (Fluorometric)
K981-100	Biovision	each	EUR 705.6

PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
K456-100	Biovision	each	EUR 574.8

Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
K767-100	Biovision	each	EUR 914.4

DNAse I Activity Assay Kit (Fluorometric)
K429-100	Biovision	each	EUR 836.4

Chymotrypsin Activity Assay Kit (Fluorometric)
K352-100	Biovision	each	EUR 588

Lipoxygenase Activity Assay Kit (Fluorometric)
K978-100	Biovision	each	EUR 783.6

Factor IXa Activity Assay Kit (Fluorometric)
K364-100	Biovision	each	EUR 588

?-Glucuronidase Activity Assay Kit (Fluorometric)
K514-100	Biovision	each	EUR 744

Xanthine Oxidase -XOD- Activity Fluorometric Assay Kit
E-BC-F019-500Assays	Elabscience Biotech	500 Assays	EUR 960
Description: Enzyme Activity

Xanthine Oxidase -XOD- Activity Fluorometric Assay Kit
E-BC-F019-96T	Elabscience Biotech	96T	EUR 32
Description: Enzyme Activity

Xanthine Oxidase -XOD- Activity Fluorometric Assay Kit
E-BC-F019-each	Elabscience Biotech	each	Ask for price
Description: Enzyme Activity

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit
MBS2567665-500Tests	MyBiosource	500Tests	EUR 880

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit
MBS2567665-5x100Tests	MyBiosource	5x100Tests	EUR 4055

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit
MBS2567665-5x96Test	MyBiosource	5x96Test	EUR 1665

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit
MBS2567665-96Test	MyBiosource	96Test	EUR 365

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit
MBS2567665-96Tests	MyBiosource	96Tests	EUR 365

Deubiquitinase Activity Assay Kit (Fluorometric)
K485-100	Biovision	each	EUR 639.6

Cathepsin E Activity Assay Kit (Fluorometric)
K165-100	Biovision	each	EUR 764.4

Factor VIIIa Activity Assay Kit (Fluorometric)
K358-100	Biovision	each	EUR 646.8

TEV Protease Activity Assay Kit (Fluorometric)
K842-100	Biovision	each	EUR 601.2

Chitotriosidase Activity Assay Kit (Fluorometric)
K512-100	Biovision	each	EUR 744

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric
MBS168350-100Assays	MyBiosource	100Assays	EUR 490

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric
MBS168350-5x100Assays	MyBiosource	5x100Assays	EUR 2235

Lysyl Oxidase Activity Assay Kit (Fluorometric)
K928-100	Biovision	each	EUR 639.6

Enolase Activity Colorimetric/Fluorometric Assay Kit
K691-100	Biovision	each	EUR 952.8

Fluorometric Proteasome 20S Activity Assay Kit
K2242-100	ApexBio	100T	EUR 444
Description: Cell Biology|Enzyme Activity Detection

?-L-Fucosidase Activity Assay Kit (Fluorometric)
K542-100	Biovision	each	EUR 627.6

Alkaline Phosphatase -ALP- Activity Assay Kit -PNPP method-
E-BC-K009-M-500Assays	Elabscience Biotech	500 Assays	EUR 400
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit -PNPP method-
E-BC-K009-M-96T	Elabscience Biotech	96T	EUR 15
Description: Enzyme Activity

Alkaline Phosphatase -ALP- Activity Assay Kit -PNPP method-
E-BC-K009-M-each	Elabscience Biotech	each	Ask for price
Description: Enzyme Activity

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
MBS2567975-500Assays	MyBiosource	500Assays	EUR 435

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
MBS2567975-500Tests	MyBiosource	500Tests	EUR 435

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
MBS2567975-5x100Tests	MyBiosource	5x100Tests	EUR 1975

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
MBS2567975-96Test	MyBiosource	96Test	EUR 250

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
MBS2567975-96Tests	MyBiosource	96Tests	EUR 250

Methyltransferase Activity Assay Kit (Fluorometric)
K521-100	Biovision	each	EUR 679.2

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
K807-100	Biovision	each	EUR 1038

Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit
K758-100	Biovision	each	EUR 732

Catalase Activity Colorimetric/Fluorometric Assay Kit
K2177-100	ApexBio	100 assays	EUR 675.2
Description: Kits|Metabolism Kit#Metabolism Kit|Oxidative Stress Kit#Kits

Catalase Activity Colorimetric/Fluorometric Assay Kit
K773-100	Biovision	each	EUR 502.8

Diamine Oxidase Activity Assay Kit (Fluorometric)
K496-100	Biovision	each	EUR 679.2

?-Secretase (BACE1) Activity Assay Kit (Fluorometric)
K388-100	Biovision	each	EUR 724.8

The first key inhibit the main activity of fluorescein by phosphate. The second key is almost completely disable fluorescein by forming nanoparticles coordination (CNPS) through self-assembly of cerium chloride and fluorescein diphosphate (FDP).