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Synthesis, characterization, in vitro tissue-nonspecific alkaline phosphatase (TNAP) and intestinal alkaline phosphatase (IAP) inhibition studies and computational evaluation of novel thiazole derivatives

Synthesis, characterization, in vitro tissue-nonspecific alkaline phosphatase (TNAP) and intestinal alkaline phosphatase (IAP) inhibition studies and computational evaluation of novel thiazole derivatives

alkaline phosphatase (AP) is a homodimeric enzyme class that has the ability dephosphorylation physiological. AP catalyzes the hydrolysis of monoesters into phosphoric acid which in turn catalyzes transfosforilasi. Thiazoles are nitrogen and sulfur-containing aromatic heterocycles AP regarded as effective inhibitors.

In this context, research papers currently presenting a successful synthesis, spectroscopic characterization and in vitro alkaline phosphatase inhibition potential new thiazole derivatives. Structure activity relationship and molecular docking studies conducted to determine the binding mode of the compound screened by the target site tissue non-specific alkaline phosphatase (h-TNAP) and intestinal alkaline phosphatase (h-IAP). 5e compound was found to be a potent inhibitor of h-TNAP with IC50 of 0.17 ± 0.01 pM.

In addition, the compounds 5a and 5i are found to be very selective about the h-TNAP with IC50 values ​​of 0.25 ± 0.01 and 0.21 ± 0.02 pM pM, respectively. In the case of h-IAP inhibitor compound 5f is the most potent with IC50 value of 1.33 ± 0.10 pM. The most active compound is a molecular docking studies on the resort-TNAP h and h-IAP binding interactions to explore the possibility of enzyme-ligand complex. performed molecular dynamics simulations to investigate the overall stability of the protein in the apo and holo country.

Breast cancer is often characterized by calcification in mammography. Mechanisms for calcifications in breast cancer is not fully known. Understanding these mechanisms will improve diagnostic accuracy. Here, we show that calcification occurs and that alkaline phosphatase enzyme activity increased MDA-MB-231 cells were cultured using a cocktail containing osteogenic media.

Transcript microarray analysis showed that PI3K-Akt signaling were significantly involved, with the recruitment of placental alkaline phosphatase. Calcification and alkaline phosphatase enzyme activity was suppressed by placental alkaline phosphatase silencing using small interfering RNA. Inhibition of PI3K-Akt signaling pathway is pressed phospho-c-Jun and placental alkaline phosphatase and resulted in the absence of calcification.

CEVO oxidase catalysis combines 4-mimetic with hexametaphosphate ion induced aggregation for sensing electrostatic photometry alkaline phosphatase activity

In this study, a new alkaline phosphatase (ALP) activity colorimetric assay proposed by integrating the catalytic characteristics oxidase-mimicking nanoparticles CeVO4 with hexametaphosphate ion (HMPi) mediated electrostatic aggregation.

The CeVO4 nanoparticles showed excellent catalytic ability oxidase-mimetic to promote 3,3 ’, 5,5′-tetramethylbenzidine (TMB) oxidation TMBox, offers significant changes from colorless to blue. After a small amount HMPi added, a strong electrostatic interaction between the negatively charged species HMPi and positively charged TMBox leads aggregation of the last product, produce eggplant HMPi-TMBox clot.

After the clot is filtered out, it turns out the reaction solution becomes almost colorless. When the ALP used to hydrolyze HMPi species in advance, electrostatic aggregation process is highly controllable, so that it retains the blue color CeVO4 catalyzed TMB solution.

Alkaline Phosphatase antibody
10R-10561 100 ug
EUR 420
Description: Mouse monoclonal Alkaline Phosphatase antibody

Alkaline Phosphatase Antibody
48608 100ul
EUR 499

Alkaline Phosphatase Antibody
48608-100ul 100ul
EUR 399.6

Alkaline Phosphatase Antibody
48608-50ul 50ul
EUR 286.8

Alkaline phosphatase Antibody
CSB-PA001633ZA01EYG-02mg 0.2mg Ask for price
Description: Recombinant Prevotella intermedia Alkaline phosphatase protein

Alkaline phosphatase Antibody
CSB-PA001633ZA01EYG-10mg 10mg Ask for price
Description: Recombinant Prevotella intermedia Alkaline phosphatase protein

Alkaline Phosphatase Antibody
DF6225 200ul
EUR 420

Alkaline Phosphatase Antibody
DF6225-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Alkaline Phosphatase Antibody
DF6225-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Alkaline Phosphatase Antibody
BF0179 200ul
EUR 540

Alkaline Phosphatase Antibody
BF0179-100ul 100ul
EUR 210
Description: WB,IHC,ELISA,FACS

Alkaline Phosphatase Antibody
BF0179-200ul 200ul
EUR 270
Description: WB,IHC,ELISA,FACS

Alkaline Phosphatase Antibody
BF0179-50ul 50ul
EUR 150
Description: WB,IHC,ELISA,FACS

Alkaline Phosphatase Antibody
DF4722 200ul
EUR 420

Alkaline Phosphatase Antibody
DF4722-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Alkaline Phosphatase Antibody
DF4722-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Alkaline Phosphatase Antibody
GWB-45EB8D 0.2 mg Ask for price

Alkaline Phosphatase Antibody
GWB-4EC464 0.2 mg Ask for price

Alkaline Phosphatase Antibody
GWB-5FDDCB 1 ml Ask for price

Alkaline Phosphatase Antibody
E80806-3 100μl
EUR 225
Description: Available in various conjugation types.

Alkaline Phosphatase Antibody
GWB-B4895D 0.2 mg Ask for price

Alkaline Phosphatase Antibody
GWB-694130 0.2 mg Ask for price

Alkaline Phosphatase Antibody
GWB-7C9507 50 mg Ask for price

Alkaline Phosphatase Antibody
GWB-8A4C93 0.2 mg Ask for price

Alkaline Phosphatase Antibody
GWB-3A419E 1 mg Ask for price

Alkaline Phosphatase antibody
MBS5310684-01mg 0.1mg
EUR 1235

Alkaline Phosphatase antibody
MBS5310684-5x01mg 5x0.1mg
EUR 5415

Alkaline Phosphatase Antibody
MBS5311788-01mL 0.1mL
EUR 1070

Alkaline Phosphatase Antibody
MBS5311788-5x01mL 5x0.1mL
EUR 4655

Alkaline Phosphatase Antibody
MBS5312378-01mL 0.1mL
EUR 1070

Alkaline Phosphatase Antibody
MBS5312378-5x01mL 5x0.1mL
EUR 4655

Alkaline Phosphatase antibody
MBS531248-1mg 1mg
EUR 255

Alkaline Phosphatase antibody
MBS531248-5x1mg 5x1mg
EUR 1000

Alkaline Phosphatase antibody
MBS530176-1mg 1mg
EUR 305

Alkaline Phosphatase antibody
MBS530176-5x1mg 5x1mg
EUR 1230

Alkaline Phosphatase antibody
MBS531433-1mg 1mg
EUR 2435

Alkaline Phosphatase antibody
MBS531433-5x1mg 5x1mg
EUR 10820

Alkaline phosphatase Antibody
MBS7137922-INQUIRE INQUIRE Ask for price

Alkaline Phosphatase Antibody
MBS850594-01mL 0.1mL
EUR 345

Alkaline Phosphatase Antibody
MBS850594-01mLAF405L 0.1mL(AF405L)
EUR 565

Alkaline Phosphatase Antibody
MBS850594-01mLAF405S 0.1mL(AF405S)
EUR 565

Alkaline Phosphatase Antibody
MBS850594-01mLAF610 0.1mL(AF610)
EUR 565

Alkaline Phosphatase Antibody
MBS850594-01mLAF635 0.1mL(AF635)
EUR 565

Alkaline Phosphatase antibody
MBS831504-1mg 1mg
EUR 345

Alkaline Phosphatase antibody
MBS831504-5x1mg 5x1mg
EUR 1410

Alkaline Phosphatase Antibody
MBS857501-01mL 0.1mL
EUR 310

Alkaline Phosphatase Antibody
MBS8564677-01mL 0.1mL
EUR 305

Alkaline Phosphatase Antibody
MBS8564677-01mLAF405L 0.1mL(AF405L)
EUR 565

Alkaline Phosphatase Antibody
MBS8564677-01mLAF405S 0.1mL(AF405S)
EUR 565

Alkaline Phosphatase Antibody
MBS8564677-01mLAF610 0.1mL(AF610)
EUR 565

Alkaline Phosphatase Antibody
MBS8564677-01mLAF635 0.1mL(AF635)
EUR 565

Alkaline Phosphatase Antibody
MBS8565957-01mL 0.1mL
EUR 310

Alkaline Phosphatase Antibody
MBS9435460-005mL 0.05mL
EUR 325

Alkaline Phosphatase Antibody
MBS9435460-01mL 0.1mL
EUR 435

Alkaline Phosphatase Antibody
MBS9435460-5x01mL 5x0.1mL
EUR 1810

Alkaline Phosphatase (AP) Antibody
abx020685-1mg 1 mg
EUR 526.8

Alkaline Phosphatase Antibody (BAP)
GWB-7958FE 1 ml Ask for price

Calf Alkaline Phosphatase Antibody
GWB-87097C 1 ml Ask for price

Bone Alkaline Phosphatase Antibody
GWB-35D182 0.1 mg Ask for price

Placental alkaline phosphatase Antibody
abx139037-01mg 0.1 mg
EUR 393.6

Placental alkaline phosphatase Antibody
49615 100ul
EUR 499

Placental alkaline phosphatase Antibody
49615-100ul 100ul
EUR 399.6

Placental alkaline phosphatase Antibody
49615-50ul 50ul
EUR 286.8

Placental Alkaline Phosphatase Antibody
GWB-5B15F7 0.5 ml Ask for price

Placental Alkaline Phosphatase Antibody
GWB-154E9E 1 ml Ask for price

Placental Alkaline Phosphatase Antibody
GWB-FC996F 0.2 mg Ask for price

Placental Alkaline Phosphatase Antibody
MBS9436358-005mL 0.05mL
EUR 325

Placental Alkaline Phosphatase Antibody
MBS9436358-01mL 0.1mL
EUR 435

Placental Alkaline Phosphatase Antibody
MBS9436358-5x01mL 5x0.1mL
EUR 1810

Alkaline Phosphatase (ALP) Antibody
abx038456-100ug 100 ug
EUR 469.2

Alkaline Phosphatase (ALP) Antibody
20-abx121265
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

Alkaline Phosphatase (ALP) Antibody
abx139037-100l 100 µl
EUR 250

Alkaline Phosphatase (ALP) Antibody
abx139037-500l 500 µl Ask for price

Alkaline Phosphatase (ALP) Antibody
abx175316-1096tests 10 × 96 tests
EUR 725

Alkaline Phosphatase (ALP) Antibody
20-abx175316
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  • Ask for price
  • 200 ug
  • 1 mg

Alkaline Phosphatase (ALP) Antibody
abx175316-596tests 5 × 96 tests
EUR 337.5

Alkaline Phosphatase (ALP) Antibody
abx175316-96tests 96 tests
EUR 262.5

Alkaline Phosphatase (ALP) Antibody
20-abx175317
  • Ask for price
  • Ask for price
  • 200 ug
  • 1 mg

BAD Antibody (Alkaline Phosphatase)
abx449461-100ug 100 ug
EUR 727.2

BAD Antibody (Alkaline Phosphatase)
abx449479-100ug 100 ug
EUR 727.2

GST Antibody (Alkaline Phosphatase)
abx448867-100ug 100 ug
EUR 727.2

CBP Antibody (Alkaline Phosphatase)
abx449065-100ug 100 ug
EUR 727.2

CBP Antibody (Alkaline Phosphatase)
abx449083-100ug 100 ug
EUR 727.2

Alkaline Phosphatase(V17.1) Antibody
BNC800141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF680 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC800141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF680 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC810141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF680R conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC810141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF680R conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC430141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF543 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC430141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF543 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC610141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF660R conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC610141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF660R conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCA0141-250 250uL
EUR 459.6
Description: Primary antibody against Alkaline Phosphatase(V17.1), APC conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC700141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF770 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC700141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF770 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCH0141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCH0141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCP0141-250 250uL
EUR 459.6
Description: Primary antibody against Alkaline Phosphatase(V17.1), PerCP conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCR0141-250 250uL
EUR 459.6
Description: Primary antibody against Alkaline Phosphatase(V17.1), RPE conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCAP0141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNCAP0141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC550141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF555 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC550141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF555 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC050141-100 100uL
EUR 238.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF405M conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(V17.1) Antibody
BNC050141-500 500uL
EUR 652.8
Description: Primary antibody against Alkaline Phosphatase(V17.1), CF405M conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase (ALP) Antibody
MBS569785-1mg 1mg
EUR 300

Alkaline Phosphatase (ALP) Antibody
MBS569785-5x1mg 5x1mg
EUR 1160

RYK Antibody: Alkaline Phosphatase
MBS8000760-01mg 0.1mg
EUR 560

RYK Antibody: Alkaline Phosphatase
MBS8000760-5x01mg 5x0.1mg
EUR 1995

CBP Antibody: Alkaline Phosphatase
MBS8000958-01mg 0.1mg
EUR 560

CBP Antibody: Alkaline Phosphatase
MBS8000958-5x01mg 5x0.1mg
EUR 1995

CBP Antibody: Alkaline Phosphatase
MBS8000976-01mg 0.1mg
EUR 560

CBP Antibody: Alkaline Phosphatase
MBS8000976-5x01mg 5x0.1mg
EUR 1995

BAD Antibody: Alkaline Phosphatase
MBS8001354-01mg 0.1mg
EUR 560

BAD Antibody: Alkaline Phosphatase
MBS8001354-5x01mg 5x0.1mg
EUR 1995

BAD Antibody: Alkaline Phosphatase
MBS8001372-01mg 0.1mg
EUR 560

BAD Antibody: Alkaline Phosphatase
MBS8001372-5x01mg 5x0.1mg
EUR 1995

AKT Antibody: Alkaline Phosphatase
MBS8001642-01mg 0.1mg
EUR 560

AKT Antibody: Alkaline Phosphatase
MBS8001642-5x01mg 5x0.1mg
EUR 1995

EGF Antibody: Alkaline Phosphatase
MBS8001714-01mg 0.1mg
EUR 560

EGF Antibody: Alkaline Phosphatase
MBS8001714-5x01mg 5x0.1mg
EUR 1995

Tau Antibody: Alkaline Phosphatase
MBS8002075-01mg 0.1mg
EUR 560

Tau Antibody: Alkaline Phosphatase
MBS8002075-5x01mg 5x0.1mg
EUR 1995

Tau Antibody: Alkaline Phosphatase
MBS8002093-01mg 0.1mg
EUR 560

Tau Antibody: Alkaline Phosphatase
MBS8002093-5x01mg 5x0.1mg
EUR 1995

Tau Antibody: Alkaline Phosphatase
MBS8005948-01mg 0.1mg
EUR 560

Tau Antibody: Alkaline Phosphatase
MBS8005948-5x01mg 5x0.1mg
EUR 1995

p23 Antibody: Alkaline Phosphatase
MBS803451-01mg 0.1mg
EUR 425

p23 Antibody: Alkaline Phosphatase
MBS803451-5x01mg 5x0.1mg
EUR 1650

RAS Antibody: Alkaline Phosphatase
MBS804960-01mg 0.1mg
EUR 515

RAS Antibody: Alkaline Phosphatase
MBS804960-5x01mg 5x0.1mg
EUR 1775

BVR Antibody: Alkaline Phosphatase
MBS805341-01mg 0.1mg
EUR 565

BVR Antibody: Alkaline Phosphatase
MBS805341-5x01mg 5x0.1mg
EUR 2000

p38 Antibody: Alkaline Phosphatase
MBS805793-01mL 0.1mL
EUR 460

p38 Antibody: Alkaline Phosphatase
MBS805793-5x01mL 5x0.1mL
EUR 1525

BVR Antibody: Alkaline Phosphatase
MBS805804-01mg 0.1mg
EUR 565

BVR Antibody: Alkaline Phosphatase
MBS805804-5x01mg 5x0.1mg
EUR 2000

According to the new sensing strategy, analysis of a highly selective and sensitive ALP activity is realized, providing a wide detection range 1-210 U / L and a detection limit of 0.68 U / L. accurate measurement of ALP activity in serum matrix also clinically validated, demonstrate the practicality assay proposed in the practical application.

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